Overview
Kisspeptin refers to a family of peptides (KP-54, KP-14, KP-13, KP-10) encoded by the KISS1 gene, which are essential regulators of the hypothalamic-pituitary-gonadal (HPG) axis. Named after Hershey, Pennsylvania (where they were discovered), they function as master regulators of puberty onset, reproductive cycling, and fertility. KP-54 is the predominant form in humans. Research shows kisspeptin may also link reproductive function with metabolic status, making it a target for both fertility disorders and metabolic disease.
Mechanism of Action
Kisspeptin binds to KISS1R (GPR54), a G-protein coupled receptor coupled to Gq/11α, which activates phospholipase C and drives pulsatile GnRH release from hypothalamic neurons. This triggers downstream LH and FSH secretion from the pituitary, driving gonadal steroid production. KP54 bolus infusion in healthy women during the follicular phase causes pulsatile LH secretion. KP also acts peripherally in the ovary, testis, uterus, and pancreas, influencing local reproductive function and insulin secretion.
Potential Benefits
- Restoration of pulsatile GnRH/LH/FSH secretion in infertility
- Potential treatment of hypothalamic amenorrhea
- Ovarian stimulation protocol adjunct
- Puberty induction in idiopathic hypogonadotropic hypogonadism
- Potential improvement of sexual desire (central effects)
- Linkage between metabolic status and reproductive function
- Cryoprotectant research in oocyte vitrification
Dosage Protocols
The following reflects doses used in published research studies. This is not medical advice. Consult a qualified healthcare professional.
| Typical Range | 100-300 mcg subcutaneous (Kisspeptin-10) |
| Beginner | 100 mcg subcutaneously |
| Intermediate | 150-200 mcg subcutaneously |
| Advanced | 300 mcg subcutaneously or clinical IV infusion protocol |
| Cycle Duration | 4-8 weeks; some use intermittent (2-3x/week) |
| Cycle Off | 4 weeks |
Kisspeptin-54 and Kisspeptin-10 (C-terminal decapeptide) are both used; Kisspeptin-10 is more commonly available and studied. Pulsatile dosing preserves LH sensitivity. Continuous high-dose administration can paradoxically suppress GnRH via receptor desensitization.
Use our Reconstitution Calculator to determine exact syringe units for your protocol.
Routes of Administration
Subcutaneous Injection High
Preferred research route; produces pulsatile LH and FSH surges; half-life ~29 minutes for Kisspeptin-54
Intramuscular Injection High
Alternative to subcutaneous; comparable LH stimulation profile
Read our full Routes of Administration Guide for detailed comparison of all delivery methods.
Stacking Protocols
Popular research stacks involving Kisspeptin:
HPG Axis Optimization Stack
Testosterone axis stimulation and central sexual arousal
Kisspeptin drives upstream GnRH pulsatility and LH/testosterone elevation; PT-141 activates melanocortin pathways; complementary mechanisms.
Fertility Support Stack
Hypothalamic-pituitary-gonadal axis stimulation for testosterone and fertility restoration
Used off-label in post-TRT recovery; Kisspeptin reactivates hypothalamic GnRH pulse generation; Gonadorelin provides direct GnRH receptor stimulation.
Explore our complete Peptide Stacking Guide for more combinations and safety considerations.
Reconstitution
| Typical Vial Size | 2mg, 5mg (Kisspeptin-10), 10mg (Kisspeptin-54) |
|---|---|
| BAC Water | 1-2ml per 2mg vial |
| Storage | Refrigerate at 2-8°C after reconstitution |
| Shelf Life | 28 days refrigerated |
Need exact syringe measurements?
Amino Acid Sequence
KP-54: YNWNSFGLRF-amide and additional 44 N-terminal residues; KP-10: Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH2
Side Effects & Safety
- Generally well-tolerated in clinical studies
- Nausea at high doses
- Hot flashes
- Potential ovarian hyperstimulation with fertility protocols
Synergistic Compounds
The following compounds have been studied alongside Kisspeptin for potential complementary or synergistic effects:
Learn More
References & Further Reading
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